Review





Similar Products

counterstaining with dapi  (Dojindo Labs)
96
Dojindo Labs counterstaining with dapi
Activation of Wnt/β-catenin signaling enhances STB differentiation. (A) A scheme of the culture conditions. Undifferentiated macTSCs were cultured with [CHIR(+)] or without [CHIR(−)] CHIR99021 for 3 days. Some cells were induced to differentiate in the medium containing dbcAMP [CHIR(−) cAMP and CHIR(+) cAMP] for another 6 days. (B) The relative expression level of a vCTB marker, TEAD4 . (C) Phase contrast images of cells on day 9. Multinucleated STB is outlined by a red line. Scale bar = 100 μm. (D, G) The relative expression levels of STB (D) and EVT (G) markers. For C, D, and G, the mean values (±SD) of technical triplicates of biological duplicates, normalized by the expression of ACTB , are shown relative to those in CHIR(−) (arbitrarily set as 1). Different letters represent significant differences ( p < 0.05). (E) Detection of multinucleated STBs by immunofluorescence for E-cadherin, CG, and <t>DAPI</t> staining. Scale bar = 100 μm. (F) The mean intensity ± SD (%) of STB nuclei calculated from four randomly chosen fields of biological duplicates. Different letters represent significant differences ( p < 0.05). Note that error bars got larger as the STB ratio increased, likely because the larger the STBs formed, the sparser the distribution of STBs became so that some of the randomly selected fields contained very few STBs. (H) Detection of gelatinase activities of MMP2 and MMP9 by gelatin zymography.
Counterstaining With Dapi, supplied by Dojindo Labs, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/counterstaining with dapi/product/Dojindo Labs
Average 96 stars, based on 1 article reviews
counterstaining with dapi - by Bioz Stars, 2026-05
96/100 stars
  Buy from Supplier
dapi counterstaining solution  (Vector Laboratories)
98
Vector Laboratories dapi counterstaining solution
Activation of Wnt/β-catenin signaling enhances STB differentiation. (A) A scheme of the culture conditions. Undifferentiated macTSCs were cultured with [CHIR(+)] or without [CHIR(−)] CHIR99021 for 3 days. Some cells were induced to differentiate in the medium containing dbcAMP [CHIR(−) cAMP and CHIR(+) cAMP] for another 6 days. (B) The relative expression level of a vCTB marker, TEAD4 . (C) Phase contrast images of cells on day 9. Multinucleated STB is outlined by a red line. Scale bar = 100 μm. (D, G) The relative expression levels of STB (D) and EVT (G) markers. For C, D, and G, the mean values (±SD) of technical triplicates of biological duplicates, normalized by the expression of ACTB , are shown relative to those in CHIR(−) (arbitrarily set as 1). Different letters represent significant differences ( p < 0.05). (E) Detection of multinucleated STBs by immunofluorescence for E-cadherin, CG, and <t>DAPI</t> staining. Scale bar = 100 μm. (F) The mean intensity ± SD (%) of STB nuclei calculated from four randomly chosen fields of biological duplicates. Different letters represent significant differences ( p < 0.05). Note that error bars got larger as the STB ratio increased, likely because the larger the STBs formed, the sparser the distribution of STBs became so that some of the randomly selected fields contained very few STBs. (H) Detection of gelatinase activities of MMP2 and MMP9 by gelatin zymography.
Dapi Counterstaining Solution, supplied by Vector Laboratories, used in various techniques. Bioz Stars score: 98/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/dapi counterstaining solution/product/Vector Laboratories
Average 98 stars, based on 1 article reviews
dapi counterstaining solution - by Bioz Stars, 2026-05
98/100 stars
  Buy from Supplier
dapi counterstain solution  (Thermo Fisher)
90
Thermo Fisher dapi counterstain solution
Activation of Wnt/β-catenin signaling enhances STB differentiation. (A) A scheme of the culture conditions. Undifferentiated macTSCs were cultured with [CHIR(+)] or without [CHIR(−)] CHIR99021 for 3 days. Some cells were induced to differentiate in the medium containing dbcAMP [CHIR(−) cAMP and CHIR(+) cAMP] for another 6 days. (B) The relative expression level of a vCTB marker, TEAD4 . (C) Phase contrast images of cells on day 9. Multinucleated STB is outlined by a red line. Scale bar = 100 μm. (D, G) The relative expression levels of STB (D) and EVT (G) markers. For C, D, and G, the mean values (±SD) of technical triplicates of biological duplicates, normalized by the expression of ACTB , are shown relative to those in CHIR(−) (arbitrarily set as 1). Different letters represent significant differences ( p < 0.05). (E) Detection of multinucleated STBs by immunofluorescence for E-cadherin, CG, and <t>DAPI</t> staining. Scale bar = 100 μm. (F) The mean intensity ± SD (%) of STB nuclei calculated from four randomly chosen fields of biological duplicates. Different letters represent significant differences ( p < 0.05). Note that error bars got larger as the STB ratio increased, likely because the larger the STBs formed, the sparser the distribution of STBs became so that some of the randomly selected fields contained very few STBs. (H) Detection of gelatinase activities of MMP2 and MMP9 by gelatin zymography.
Dapi Counterstain Solution, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/dapi counterstain solution/product/Thermo Fisher
Average 90 stars, based on 1 article reviews
dapi counterstain solution - by Bioz Stars, 2026-05
90/100 stars
  Buy from Supplier
dapi-based mounting solution dapi i counterstain 1000 ng/ml  (Abbott Laboratories)
90
Abbott Laboratories dapi-based mounting solution dapi i counterstain 1000 ng/ml
Activation of Wnt/β-catenin signaling enhances STB differentiation. (A) A scheme of the culture conditions. Undifferentiated macTSCs were cultured with [CHIR(+)] or without [CHIR(−)] CHIR99021 for 3 days. Some cells were induced to differentiate in the medium containing dbcAMP [CHIR(−) cAMP and CHIR(+) cAMP] for another 6 days. (B) The relative expression level of a vCTB marker, TEAD4 . (C) Phase contrast images of cells on day 9. Multinucleated STB is outlined by a red line. Scale bar = 100 μm. (D, G) The relative expression levels of STB (D) and EVT (G) markers. For C, D, and G, the mean values (±SD) of technical triplicates of biological duplicates, normalized by the expression of ACTB , are shown relative to those in CHIR(−) (arbitrarily set as 1). Different letters represent significant differences ( p < 0.05). (E) Detection of multinucleated STBs by immunofluorescence for E-cadherin, CG, and <t>DAPI</t> staining. Scale bar = 100 μm. (F) The mean intensity ± SD (%) of STB nuclei calculated from four randomly chosen fields of biological duplicates. Different letters represent significant differences ( p < 0.05). Note that error bars got larger as the STB ratio increased, likely because the larger the STBs formed, the sparser the distribution of STBs became so that some of the randomly selected fields contained very few STBs. (H) Detection of gelatinase activities of MMP2 and MMP9 by gelatin zymography.
Dapi Based Mounting Solution Dapi I Counterstain 1000 Ng/Ml, supplied by Abbott Laboratories, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/dapi-based mounting solution dapi i counterstain 1000 ng/ml/product/Abbott Laboratories
Average 90 stars, based on 1 article reviews
dapi-based mounting solution dapi i counterstain 1000 ng/ml - by Bioz Stars, 2026-05
90/100 stars
  Buy from Supplier
4',6-diamidine-2-phenylindole (dapi) counterstain solution  (Millipore)
90
Millipore 4',6-diamidine-2-phenylindole (dapi) counterstain solution
Activation of Wnt/β-catenin signaling enhances STB differentiation. (A) A scheme of the culture conditions. Undifferentiated macTSCs were cultured with [CHIR(+)] or without [CHIR(−)] CHIR99021 for 3 days. Some cells were induced to differentiate in the medium containing dbcAMP [CHIR(−) cAMP and CHIR(+) cAMP] for another 6 days. (B) The relative expression level of a vCTB marker, TEAD4 . (C) Phase contrast images of cells on day 9. Multinucleated STB is outlined by a red line. Scale bar = 100 μm. (D, G) The relative expression levels of STB (D) and EVT (G) markers. For C, D, and G, the mean values (±SD) of technical triplicates of biological duplicates, normalized by the expression of ACTB , are shown relative to those in CHIR(−) (arbitrarily set as 1). Different letters represent significant differences ( p < 0.05). (E) Detection of multinucleated STBs by immunofluorescence for E-cadherin, CG, and <t>DAPI</t> staining. Scale bar = 100 μm. (F) The mean intensity ± SD (%) of STB nuclei calculated from four randomly chosen fields of biological duplicates. Different letters represent significant differences ( p < 0.05). Note that error bars got larger as the STB ratio increased, likely because the larger the STBs formed, the sparser the distribution of STBs became so that some of the randomly selected fields contained very few STBs. (H) Detection of gelatinase activities of MMP2 and MMP9 by gelatin zymography.
4',6 Diamidine 2 Phenylindole (Dapi) Counterstain Solution, supplied by Millipore, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/4',6-diamidine-2-phenylindole (dapi) counterstain solution/product/Millipore
Average 90 stars, based on 1 article reviews
4',6-diamidine-2-phenylindole (dapi) counterstain solution - by Bioz Stars, 2026-05
90/100 stars
  Buy from Supplier
counterstain solution  (Vector Laboratories)
98
Vector Laboratories counterstain solution
Activation of Wnt/β-catenin signaling enhances STB differentiation. (A) A scheme of the culture conditions. Undifferentiated macTSCs were cultured with [CHIR(+)] or without [CHIR(−)] CHIR99021 for 3 days. Some cells were induced to differentiate in the medium containing dbcAMP [CHIR(−) cAMP and CHIR(+) cAMP] for another 6 days. (B) The relative expression level of a vCTB marker, TEAD4 . (C) Phase contrast images of cells on day 9. Multinucleated STB is outlined by a red line. Scale bar = 100 μm. (D, G) The relative expression levels of STB (D) and EVT (G) markers. For C, D, and G, the mean values (±SD) of technical triplicates of biological duplicates, normalized by the expression of ACTB , are shown relative to those in CHIR(−) (arbitrarily set as 1). Different letters represent significant differences ( p < 0.05). (E) Detection of multinucleated STBs by immunofluorescence for E-cadherin, CG, and <t>DAPI</t> staining. Scale bar = 100 μm. (F) The mean intensity ± SD (%) of STB nuclei calculated from four randomly chosen fields of biological duplicates. Different letters represent significant differences ( p < 0.05). Note that error bars got larger as the STB ratio increased, likely because the larger the STBs formed, the sparser the distribution of STBs became so that some of the randomly selected fields contained very few STBs. (H) Detection of gelatinase activities of MMP2 and MMP9 by gelatin zymography.
Counterstain Solution, supplied by Vector Laboratories, used in various techniques. Bioz Stars score: 98/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/counterstain solution/product/Vector Laboratories
Average 98 stars, based on 1 article reviews
counterstain solution - by Bioz Stars, 2026-05
98/100 stars
  Buy from Supplier

Image Search Results


Activation of Wnt/β-catenin signaling enhances STB differentiation. (A) A scheme of the culture conditions. Undifferentiated macTSCs were cultured with [CHIR(+)] or without [CHIR(−)] CHIR99021 for 3 days. Some cells were induced to differentiate in the medium containing dbcAMP [CHIR(−) cAMP and CHIR(+) cAMP] for another 6 days. (B) The relative expression level of a vCTB marker, TEAD4 . (C) Phase contrast images of cells on day 9. Multinucleated STB is outlined by a red line. Scale bar = 100 μm. (D, G) The relative expression levels of STB (D) and EVT (G) markers. For C, D, and G, the mean values (±SD) of technical triplicates of biological duplicates, normalized by the expression of ACTB , are shown relative to those in CHIR(−) (arbitrarily set as 1). Different letters represent significant differences ( p < 0.05). (E) Detection of multinucleated STBs by immunofluorescence for E-cadherin, CG, and DAPI staining. Scale bar = 100 μm. (F) The mean intensity ± SD (%) of STB nuclei calculated from four randomly chosen fields of biological duplicates. Different letters represent significant differences ( p < 0.05). Note that error bars got larger as the STB ratio increased, likely because the larger the STBs formed, the sparser the distribution of STBs became so that some of the randomly selected fields contained very few STBs. (H) Detection of gelatinase activities of MMP2 and MMP9 by gelatin zymography.

Journal: Biology of Reproduction

Article Title: Wnt signaling activation confers a syncytiotrophoblast progenitor state on trophoblast stem cells of cynomolgus monkey †

doi: 10.1093/biolre/ioae131

Figure Lengend Snippet: Activation of Wnt/β-catenin signaling enhances STB differentiation. (A) A scheme of the culture conditions. Undifferentiated macTSCs were cultured with [CHIR(+)] or without [CHIR(−)] CHIR99021 for 3 days. Some cells were induced to differentiate in the medium containing dbcAMP [CHIR(−) cAMP and CHIR(+) cAMP] for another 6 days. (B) The relative expression level of a vCTB marker, TEAD4 . (C) Phase contrast images of cells on day 9. Multinucleated STB is outlined by a red line. Scale bar = 100 μm. (D, G) The relative expression levels of STB (D) and EVT (G) markers. For C, D, and G, the mean values (±SD) of technical triplicates of biological duplicates, normalized by the expression of ACTB , are shown relative to those in CHIR(−) (arbitrarily set as 1). Different letters represent significant differences ( p < 0.05). (E) Detection of multinucleated STBs by immunofluorescence for E-cadherin, CG, and DAPI staining. Scale bar = 100 μm. (F) The mean intensity ± SD (%) of STB nuclei calculated from four randomly chosen fields of biological duplicates. Different letters represent significant differences ( p < 0.05). Note that error bars got larger as the STB ratio increased, likely because the larger the STBs formed, the sparser the distribution of STBs became so that some of the randomly selected fields contained very few STBs. (H) Detection of gelatinase activities of MMP2 and MMP9 by gelatin zymography.

Article Snippet: After nuclear counterstaining with DAPI (D523; DOJINDO, Kumamoto, Japan), the cells were mounted on glass slides with VECTASHIELD (H-1000; Vector Laboratories, Newark, CA, USA).

Techniques: Activation Assay, Cell Culture, Expressing, Marker, Immunofluorescence, Staining, Zymography

macTSCs acquire FSK responsiveness for STB differentiation by Wnt/β-catenin signaling activation. (A) A scheme of the macTSC culture conditions. Undifferentiated macTSCs were cultured with [CHIR(+)] or without [CHIR(−)] CHIR99021 for 3 days. Some cells were induced to differentiate in the medium containing forskolin (FSK) [CHIR(−) FSK and CHIR(+) FSK] for another 6 days. (B) Phase contrast images of cells on day 9. Multinucleated STB is indicated by a red line. Scale bar = 100 μm. (C) The relative expression levels of STB markers. The mean values (±SD) of technical triplicates of biological duplicates, normalized by the expression of ACTB , are shown relative to those of cells maintained under the CHIR(−) condition (arbitrarily set as 1). (D) Detection of multinucleated STB by immunofluorescence for E-cadherin, CG, and DAPI staining. Scale bar = 100 μm. (E) The mean intensity ± SD (%) of STB nuclei calculated from four randomly chosen fields of biological duplicates. Different letters represent significant differences ( p < 0.05). Note that error bars got larger as the STB ratio increased, likely because the larger the STBs formed, the sparser the distribution of STBs became so that some of the randomly selected fields contained very few STBs.

Journal: Biology of Reproduction

Article Title: Wnt signaling activation confers a syncytiotrophoblast progenitor state on trophoblast stem cells of cynomolgus monkey †

doi: 10.1093/biolre/ioae131

Figure Lengend Snippet: macTSCs acquire FSK responsiveness for STB differentiation by Wnt/β-catenin signaling activation. (A) A scheme of the macTSC culture conditions. Undifferentiated macTSCs were cultured with [CHIR(+)] or without [CHIR(−)] CHIR99021 for 3 days. Some cells were induced to differentiate in the medium containing forskolin (FSK) [CHIR(−) FSK and CHIR(+) FSK] for another 6 days. (B) Phase contrast images of cells on day 9. Multinucleated STB is indicated by a red line. Scale bar = 100 μm. (C) The relative expression levels of STB markers. The mean values (±SD) of technical triplicates of biological duplicates, normalized by the expression of ACTB , are shown relative to those of cells maintained under the CHIR(−) condition (arbitrarily set as 1). (D) Detection of multinucleated STB by immunofluorescence for E-cadherin, CG, and DAPI staining. Scale bar = 100 μm. (E) The mean intensity ± SD (%) of STB nuclei calculated from four randomly chosen fields of biological duplicates. Different letters represent significant differences ( p < 0.05). Note that error bars got larger as the STB ratio increased, likely because the larger the STBs formed, the sparser the distribution of STBs became so that some of the randomly selected fields contained very few STBs.

Article Snippet: After nuclear counterstaining with DAPI (D523; DOJINDO, Kumamoto, Japan), the cells were mounted on glass slides with VECTASHIELD (H-1000; Vector Laboratories, Newark, CA, USA).

Techniques: Activation Assay, Cell Culture, Expressing, Immunofluorescence, Staining